DNA Reagents
New England Biolabs, Inc. NEBNext End Repair Module – 100 reactions
This module is part of the original standard library prep workflow, which is longer than the NEBNext Ultra II DNA Library Prep Kit workflow, has more components, and requires more clean-up steps. It also requires higher input amounts: 1 - 5 µg of input DNA vs. 500 pg - 1 µg required for the Ultra II DNA workflow.
Sigma Aldrich Fine Chemicals Biosciences DNA Ligation Kit
Sigmas DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions. Sticky-end ligations are more efficient than blunt-end ligations these can be facilitated with the addition of PEG.
CPC Scientific (Z-Asp-Glu-Val-Asp)2-R110 1MG
Highly sensitive, photostable fluorogenic substrate for the determination of intracellular caspase-3 activity. The fluorophore rhodamine 110(Rh110) can be observed at an emission wavelength fo 535 nm with an excitation wavelength of 485 nm. With (H-Asp-Glu-Val-Asp)2-Rhodamine 110 (M-2620) the fluorescence intensity can be measured by flow cytometry, microwell plate reader, or fluorescence microscopy.SEQUENCE: (Z-Asp-Glu-Val-Asp)2-R110ONE-LETTER SEQUENCE: (Z-DEVD)2-R110MOLECULAR FORMULA: C72H78N10O27MOLECULAR WEIGHT: 1515.44STORAGE CONDITIONS: -20 5 CCAS REGISTRY NUMBER: [223538-61-2]SYNONYMS: (Z-Asp-Glu-Val-Asp)-Rhodamine 110RESEARCH AREA: ApoptosisCATEGORY: Apoptosis Related Peptides REFERENCES:Science 281, 1312 (1998)J. Biol. Chem., 276, 6974 (2001)Biochemistry 38, 13906 (1999)SKU(s): CASP-066A, CASP-066B, CASP-066C, CASP-066D
Biochain Institute Inc Total RNA - Human Adult Normal Tissue 5 Donor Pool: Heart, 50 ug/PK
BioChain's Total RNAs are isolated from a wide variety of documented human normal, diseased, and tumor tissues, mouse, rat, monkey, and plant tissues. Total RNA isolation is performed using proprietary techniques. Contamination by RNase, genomic DNA polysaccharides, and proteoglycans has been effectively eliminated. The integrity of the total RNAs is assured by checking for a ratio of greater than 1:1 between 28s and 18s ribosomal RNA. High efficiency reverse transcription using the Total RNA is demonstrated.
Zymo Research Corporation EZ DNA Methylation™ Kit (200 Rxns)
The EZ DNA Methylation Kit features a simplified procedure that streamlines bisulfite treatment of DNA. The EZ DNA Methylation Kit is based on the three-step reaction that takes place between cytosine and sodium bisulfite during which cytosine is converted into uracil. Innovative desulphonation technologies eliminate otherwise cumbersome precipitations. The kit is designed to reduce template degradation and minimize DNA loss during treatment and clean-up, while ensuring complete conversion of the DNA. Purified, converted DNA is ideal for downstream analyses including library preparation for Next-generation sequencing, PCR amplification, endonuclease digestion, sequencing, microarrays, etc. The EZ DNA Methylation Kit is recommended for use with Illumina Infinium MethylationEPIC BeadChip array.
INTACT GENOMICS INC ig-Fusion™ Cloning Enzyme Premix 100 Reactions
Intact Genomics propriety ig-Fusion™ cloning technology is a simple, rapid and highly efficient cloning kit which allows to directly clone any PCR product(s) to any linearized expression vector at any site. The PCR fragments can be generated by Intact Genomics high fidelity Pfu DNA polymerase or other high fidelity DNA polymerases, with primers having 15 to 18 bases of homology at their linear ends to where the product need to fuse. The linearized vector can be generated by PCR or restriction enzymes. The kit is so robust that multiple DNA fragments can be assembled simultaneously and cloned into one construct in a single reaction step. Cloning takes usually 10-30 min. with more than 95% cloning efficiency.Applications Clone any insert at any site within any vector Restriction enzyme and phosphatase free system Joining multiple large fragments at once Precise insertion at a desired orientation Rapid and high efficiency with > 95% positive clones
Sigma Aldrich Fine Chemicals Biosciences DEBLOCKING REAGENT 3 TRICHLOR
NC3626623 DEBLOCKING REAGENT 3 TRICHLOR